VP23.09: Oxidative stress biomarkers in amniotic fluid in fetuses with isolated congenital heart defect

Recent evidence suggests that oxidative stress (OS) plays an important role in the pathogenesis of congenital heart defect (CHD). The aim this study is to evaluate protein OS biomarkers amniotic fluid fetuses with isolated major CHD. This a retrospective including 92 samples, 50 cases diagnosis CHD and 42 controls (fetuses high risk genetic anomaly or extracardiac malformations, excluding CNS renal anomalies). All samples were collected from 2016 2020 at time performing after obtaining consent all patients. Five oxidation biomarkers, orto-tyrosine (o-tyr), metatyrosine (m-tyr), 3NO2-tyrosine (3NO2-tyr), 3I-tyrosine (3I-tyr), analysed provide preliminary results differences status between two groups, taking into account confusing factors such as gestational age (GA) amniocentesis condition maternal diabetes. Samples using Acquity-Xevo TQ system. No found groups terms GA (P = 0.8321) presence diabetes 0.182). After adjusting for GA, showed increased levels o-Tyr 0.038). other significant among rest analysed. Our data demonstrates than have biomarker fluid. Further studies evaluating greater number different its correlation postnatal outcome are warranted improve identification high-risk eventually new prevention strategies. Please note: publisher not responsible content functionality any supporting information supplied by authors. Any queries (other missing content) should be directed corresponding author article.